The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR items had been digested and cloned right into a pET21a+ vector employing NdeI and XhoI restriction enzymes. A point mutation was launched by the fast https://xp-5957890.blogchaat.com/26544488/agx51-for-dummies