Subsequent, we handled cells with distinctive concentrations of NAD+. When included for twenty-four h at 80 µM in HeLa and 400 µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Extended Data Fig. 1k,l). Furthermore, 16 µM of NAD+ was sufficient to reduce fork velocity by ~40% in HeLa cells https://nad-deficiency-symptoms-a68902.shoutmyblog.com/31047450/the-definitive-guide-to-nad-supplements-for-energy-and-metabolism